Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.082346 |
Chromosome: | chromosome_16 |
Location: | 494001 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g693204 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCTGCTGCCGCAGCTGACGAGCCAAAACA |
Internal bar code: | TCTTTCAGAGCGCGGGCTTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 563 |
LEAP-Seq percent confirming: | 98.5591 |
LEAP-Seq n confirming: | 342 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGAACTGTTGGTTCTGGTT |
Suggested primer 2: | CGCTGTTCAACGAGATTTCA |