| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082380 |
| Chromosome: | chromosome 3 |
| Location: | 270363 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144484 | CDPK10 | Calcium/calmodulin-dependent protein kinase; (1 of 4) PTHR24349//PTHR24349:SF121 - SERINE/THREONINE-PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTGTTTCACGGGCCTGGTGTGGGAGGT |
| Internal bar code: | GGGTGGTGCATAGCAAGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 444 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACGG |
| Suggested primer 2: | CACAACCCTAGCCCCTGTAA |