| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.082409 |
| Chromosome: | chromosome 12 |
| Location: | 4408403 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520850 | (1 of 1) K15153 - mediator of RNA polymerase II transcription subunit 31 (MED31, SOH1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCAGATTGACTGTCAAACCGTCCAACC |
| Internal bar code: | GCGCTAGTCGACTCATTCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 99.7674 |
| LEAP-Seq n confirming: | 5148 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTGTAAGGAAAGGCGGT |
| Suggested primer 2: | AGCAATGACTTCGCTCACCT |