Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.082442 |
Chromosome: | chromosome 10 |
Location: | 1154355 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g425800 | ORN1 | (1 of 1) 3.1.13.3 - Oligonucleotidase; Putative organellar oligoribonuclease | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGGGGGGGGCTGAGGGGACTCGGGCAC |
Internal bar code: | AAATTTACACTCATTCACTTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 360 |
LEAP-Seq percent confirming: | 99.8102 |
LEAP-Seq n confirming: | 3682 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGTGTATGCACGCGTTTGT |
Suggested primer 2: | GTAGAAGCAAGCCCTGAACG |