Insertion junction: LMJ.RY0402.082449_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.082449
Chromosome:chromosome 7
Location:2571265
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g329882 VGK1,VGKC Ion transport protein; (1 of 1) PTHR10217//PTHR10217:SF503 - VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTCTCAATAATGGTGACACGTACCATTG
Internal bar code:CTAATACAGTCGTCTTGAATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:69
LEAP-Seq percent confirming:98.75
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACTGATGGGCTGTGAGCTG
Suggested primer 2:CCATGTATGTCTACACGGCG