| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.082469 |
| Chromosome: | chromosome 6 |
| Location: | 3943643 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278188 | NUOB18 | NADH:ubiquinone oxidoreductase 10 kDa subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCAATTGCAATTGCAATTGCTCGACCC |
| Internal bar code: | CACACAGATCTAGCGGTATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 79.7737 |
| LEAP-Seq n confirming: | 564 |
| LEAP-Seq n nonconfirming: | 143 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATATGGCGGATAGGTTCCC |
| Suggested primer 2: | AACGCTGTGTCCACCTCTCT |