| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082493 |
| Chromosome: | chromosome_12 |
| Location: | 1077283 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g490850 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCTGGCGCTGGAGTCCATCGCGCAGGTGT |
| Internal bar code: | ATGGTAGTCGGGCCTAGCAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 15.2053 |
| LEAP-Seq n confirming: | 933 |
| LEAP-Seq n nonconfirming: | 5203 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTGTGGACACTAATCCC |
| Suggested primer 2: | GGGGGATTACATTCACGATG |