| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.082503 |
| Chromosome: | chromosome_1 |
| Location: | 349287 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g002200 | RPB6 | Subunit of DNA-directed RNA polymerase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGTGAAGGGCACCTTCTTCTCGCGCAGCT |
| Internal bar code: | CATACTCGGCCCGCGCGGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 11.3384 |
| LEAP-Seq n confirming: | 848 |
| LEAP-Seq n nonconfirming: | 6631 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATGGCCCAGTCCTCATA |
| Suggested primer 2: | GTATTAGGGACACGCGCATT |