| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.082527 |
| Chromosome: | chromosome 1 |
| Location: | 2869904 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017400 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGTGGCCCGGCGGTCGCTGCTGGCAGC |
| Internal bar code: | AAGGGTCAGAGCGCGCTAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 869 |
| LEAP-Seq percent confirming: | 99.9173 |
| LEAP-Seq n confirming: | 1208 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCGTTATCCGTTCATGG |
| Suggested primer 2: | CCGTCATTCTGGTGTCCTCT |