| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082533 |
| Chromosome: | chromosome 2 |
| Location: | 4247521 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098850 | NTR2 | NADPH dependent thioredoxin reductase 2; (1 of 2) PTHR22912//PTHR22912:SF183 - DISULFIDE OXIDOREDUCTASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCTGGCGGTTTCCCAGTAGGACTGCG |
| Internal bar code: | GTCGGGACGCGAGAGTTAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 92.1569 |
| LEAP-Seq n confirming: | 376 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAAACCAAACACGCAGCC |
| Suggested primer 2: | TGCAGCAGATTGAGGTGAAC |