| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.082595 |
| Chromosome: | chromosome 3 |
| Location: | 5086349 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181300 | SHKG1,SHK6 | (1 of 1) 2.5.1.19 - 3-phosphoshikimate 1-carboxyvinyltransferase / EPSP synthase; 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (EC 2.5.1.19) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCTGAGCAGGGCTCAACGCAAGCCGCG |
| Internal bar code: | GAGGGCGGTGCACACGGTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 99.5204 |
| LEAP-Seq n confirming: | 830 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAAGCAGTTTGCACCTGA |
| Suggested primer 2: | GCGGCACTACATAAGCACAA |