| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.082601 |
| Chromosome: | chromosome 7 |
| Location: | 3740554 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338000 | MCM2 | (1 of 1) K02540 - DNA replication licensing factor MCM2 (MCM2); Minichromosome maintenance protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGGTAATCATCGGCCACTGCCCGACCC |
| Internal bar code: | CCCAGGTCAAGCCGGCTATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 97.1275 |
| LEAP-Seq n confirming: | 1082 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGACGTTGGGAAACAGAT |
| Suggested primer 2: | GCAGAGAGGGAGGTGATGAG |