Insertion junction: LMJ.RY0402.082601_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.082601
Chromosome:chromosome 7
Location:3740618
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g338000 MCM2 (1 of 1) K02540 - DNA replication licensing factor MCM2 (MCM2); Minichromosome maintenance protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTGGTTTGACTTGCCGCTCACCGCAAAT
Internal bar code:GGACCGTCGGGGGGGCCACATC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:22.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GACGACGTTGGGAAACAGAT
Suggested primer 2:CCAATTGTACGAGTGCATGG