Insertion junction: LMJ.RY0402.082622_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.082622
Chromosome:chromosome 17
Location:6808671
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g744997 PNO4 FAD dependent oxidoreductase; (1 of 1) PTHR10617//PTHR10617:SF52 - ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE // OXIDOREDUCTASE/ELECTRON CARRIER-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGTGGGGGGCGTGGGGGCGAGGGGCCA
Internal bar code:TTCGACGCATACATGCTGGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:209
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ATGATACCGCAACAACACGA
Suggested primer 2:TAAAGGGGTGGACGTTTCAG