Insertion junction: LMJ.RY0402.082623_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.082623
Chromosome:chromosome_12
Location:7511271
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g555950 DHC11 Putative flagellar inner arm dynein heavy chain, novel minor typ antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):AGCGAGCAGGAGATCTGCGCGCTCATCTCA
Internal bar code:GACATGGGGGGGGATGGACAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1046
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:408
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGCAAGAAGCTAGAGACA
Suggested primer 2:GTTCCGTTCCGTGATGAAGT