| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082636 |
| Chromosome: | chromosome 4 |
| Location: | 634745 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217945 | FAD5D | Putative Linoleate desaturase; (1 of 2) K00507 - stearoyl-CoA desaturase (delta-9 desaturase) (SCD, desC) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTCGGATCCTGGAGCTGACAGGGCTGG |
| Internal bar code: | ATGCGCGGGAAGAATGGGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 97.136 |
| LEAP-Seq n confirming: | 407 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTCATGTCACTCGTCCC |
| Suggested primer 2: | AGCTCAGGACCTTACCCCAT |