| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.082679 |
| Chromosome: | chromosome 16 |
| Location: | 5893070 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677250 | TEH5 | (1 of 6) 3.1.2.2 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase; Thioesterase-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCCGTGCGGCTCTGTCATTCTGGTGTC |
| Internal bar code: | AGCAGGGACATCATACATAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 99.5611 |
| LEAP-Seq n confirming: | 1588 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTTAACCCGACGGTATG |
| Suggested primer 2: | TGCGTGCTGTTTCATTCTTC |