| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.082740 |
| Chromosome: | chromosome 13 |
| Location: | 4180895 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592200 | GLT1,GSN1 | Glutamate synthase, NADH-dependent; (1 of 1) 1.4.1.14 - Glutamate synthase (NADH) / NADH-glutamate synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACCAGCATTCCCGGCGTGTTCGCGGCG |
| Internal bar code: | GCGGCCGACGCCTGGTGAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 99.68 |
| LEAP-Seq n confirming: | 623 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCGGTACCATATGCTGT |
| Suggested primer 2: | ACCAAGGTTCGTGTCCTTTG |