| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.082753 |
| Chromosome: | chromosome 10 |
| Location: | 2978129 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440650 | (1 of 1) PTHR12176:SF18 - METHYLTRANSFERASE-LIKE PROTEIN 13 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAGTGGACTACTGACGCCACGCAGCCA |
| Internal bar code: | TTAGTGGCCTCACGATGCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 906 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATCCTGCTTCTGTTCGCC |
| Suggested primer 2: | ACAGTAGTGCATCTTGGGGG |