Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.082754 |
Chromosome: | chromosome_2 |
Location: | 7867795 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g141700 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGGGGTAGGATACGTGCCGACACCGCTCA |
Internal bar code: | TGGGATGTCGATGAGTCGTTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 655 |
LEAP-Seq percent confirming: | 81.5789 |
LEAP-Seq n confirming: | 217 |
LEAP-Seq n nonconfirming: | 49 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCCCTACCTGCCTACCTAC |
Suggested primer 2: | TGATGGCTGCTCATCTTCAC |