Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.082773 |
Chromosome: | chromosome_6 |
Location: | 3933609 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278187 | MOT4 | Predicted protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCAACCAGTCGTCAGCCTGATCGACCCGTA |
Internal bar code: | TGTGGCTCGGTGGCGGCCTGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 171 |
LEAP-Seq percent confirming: | 93.9655 |
LEAP-Seq n confirming: | 109 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAGGCCCTACACACTGGAC |
Suggested primer 2: | CAGCTAATTCCCAAACCCAA |