| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.082793 |
| Chromosome: | chromosome 6 |
| Location: | 2859089 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271900 | (1 of 1) 1.1.99.37 - Methanol dehydrogenase (nicotinoprotein) / Nicotinoprotein methanol dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCAGCACAGTGGGAAAGTGTGCGTGTT |
| Internal bar code: | TGGCTTCGACGGGGCCGCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 99.0773 |
| LEAP-Seq n confirming: | 2577 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACTGCCACACCTGAAGC |
| Suggested primer 2: | GTCATAACAGGCGCGAAGTT |