| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082856 |
| Chromosome: | chromosome 12 |
| Location: | 6223918 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGCCACCTGCTGGTGCTGTTGCGGTG |
| Internal bar code: | ACGCCGAGCACAAAGCGCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 66.5641 |
| LEAP-Seq n confirming: | 2811 |
| LEAP-Seq n nonconfirming: | 1412 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGTAGAGGCTGAAAAGC |
| Suggested primer 2: | ATCCGCTACTGCCAATCATC |