| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.082873 |
| Chromosome: | chromosome 16 |
| Location: | 57437 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g695850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCTTGCGCGACATTCAGTATAACCCTA |
| Internal bar code: | GGTTAATGTTGATATATTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 97.6772 |
| LEAP-Seq n confirming: | 4878 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATAAGGGCCTGCCTATTG |
| Suggested primer 2: | AAAGAACATGGCCTGATTGG |