Insertion junction: LMJ.RY0402.082894_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.082894
Chromosome:chromosome 10
Location:1048726
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g425251 (1 of 5) IPR008274 - Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCGCGCTGCTATTCAGTAGGACGACGAC
Internal bar code:TGTGCGTCACGTCCTGTTTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:240
LEAP-Seq percent confirming:99.5316
LEAP-Seq n confirming:425
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GATGTCAGCTCCCTACCAGG
Suggested primer 2:ATCTGGCGTCTCTGCAAGTT