| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082907 |
| Chromosome: | chromosome 9 |
| Location: | 2001427 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394450 | RBP1 | RAN binding protein, RANBP1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGTCCGCTTCCTCCTGATCATCGAAGG |
| Internal bar code: | ACACCTAGGAACCAACGAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 111 |
| LEAP-Seq percent confirming: | 76.5306 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGCTGAGGCTAAGTCGT |
| Suggested primer 2: | AGCATAGCGCAGTGTGTGTC |