| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.082909 |
| Chromosome: | chromosome 1 |
| Location: | 3796334 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g024500 | (1 of 1) K16484 - rotatin (RTTN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGCCCGGGCCCTGCTGGCGAGGCGGGC |
| Internal bar code: | TTGCACACCCGGGCCGCGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 97.0606 |
| LEAP-Seq n confirming: | 1585 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCGTATCTGGCGCTGTC |
| Suggested primer 2: | TGTGGTGAAGATCGGGGTAT |