| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.082913 |
| Chromosome: | chromosome 12 |
| Location: | 3772991 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515200 | DRC6,FAP169 | Nexin-dynein regulatory complex 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACTCGGGTAGCTGTGCGAGTGGAATATG |
| Internal bar code: | CCTGGAGTGGCAGTATAGTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 408 |
| LEAP-Seq percent confirming: | 99.8291 |
| LEAP-Seq n confirming: | 14016 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTATTCTGGCGTTGTGCC |
| Suggested primer 2: | AAGGTGGTTTACAGGCGTTG |