Insertion junction: LMJ.RY0402.082963_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.082963
Chromosome:chromosome_14
Location:2543945
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre14.g625500 FAP254,ANK10 Flagellar Associated Protein with ankyrin repeats antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TTCACTTAGCAGCAATCGAGAACCACCCCG
Internal bar code:TGGTGCGTTCTTCAAATTCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:277
LEAP-Seq percent confirming:61.3577
LEAP-Seq n confirming:1175
LEAP-Seq n nonconfirming:740
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACAACCCAGGGACACTTCT
Suggested primer 2:TCTCTGACTCTCGCTCACGA