Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.082963 |
Chromosome: | chromosome_14 |
Location: | 2543945 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g625500 | FAP254,ANK10 | Flagellar Associated Protein with ankyrin repeats | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCACTTAGCAGCAATCGAGAACCACCCCG |
Internal bar code: | TGGTGCGTTCTTCAAATTCACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 277 |
LEAP-Seq percent confirming: | 61.3577 |
LEAP-Seq n confirming: | 1175 |
LEAP-Seq n nonconfirming: | 740 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACAACCCAGGGACACTTCT |
Suggested primer 2: | TCTCTGACTCTCGCTCACGA |