| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.082994 |
| Chromosome: | chromosome 1 |
| Location: | 6853557 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049500 | COX2B | Cytochrome c oxidase subunit II, protein IIb of split subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCCCGCGGCATTTGTGTGCCGTGTGCC |
| Internal bar code: | CCCTGATCCCTCCTGATCATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 99.5085 |
| LEAP-Seq n confirming: | 4859 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTCTACGGCCAGTGCTC |
| Suggested primer 2: | GCCTAAAGCCATCTACGCAC |