Insertion junction: LMJ.RY0402.083026_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.083026
Chromosome:chromosome 6
Location:4358038
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g278267 (1 of 80) IPR003882 - Pistil-specific extensin-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTCCCCGGGGATTGGCACTGCTTCCCCA
Internal bar code:TTCGGTGGGGACCGTCGGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:322
LEAP-Seq percent confirming:85.1204
LEAP-Seq n confirming:389
LEAP-Seq n nonconfirming:68
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCCTTACTCCTCCAGCTC
Suggested primer 2:GTAGGTAATGCGGTGCCACT