| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.083043 |
| Chromosome: | chromosome 1 |
| Location: | 6787334 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048900 | GTF1 | Global Transcription factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTAGCTCCAGTGTGTGCACGGCGCGCC |
| Internal bar code: | AACGGGCGTTTGGCCGTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 67 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGAGGAGGTGAGTGACTA |
| Suggested primer 2: | AGCAAATCTGCACACACACC |