| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083046 |
| Chromosome: | chromosome 12 |
| Location: | 7223513 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558350 | (1 of 1) PF02609 - Exonuclease VII small subunit (Exonuc_VII_S) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAAGCTACCGGACATTGCTTGAAACCT |
| Internal bar code: | CGCGGGAATCGACCGGGAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 94.2581 |
| LEAP-Seq n confirming: | 22506 |
| LEAP-Seq n nonconfirming: | 1371 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCTCATCCTTGTGAGCC |
| Suggested primer 2: | AAAGGTTGTGCAGTTTTGGG |