| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.083067 |
| Chromosome: | chromosome 4 |
| Location: | 2689385 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223250 | LCIE,LCIE1 | Low-CO2 inducible protein E | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGTAAGTCTTGGGCGGGAACAGGCGCG |
| Internal bar code: | GGCCAGCTCGGGGGGGATTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1117 |
| LEAP-Seq percent confirming: | 96.5721 |
| LEAP-Seq n confirming: | 1634 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATAAGAAGGAATGCCCG |
| Suggested primer 2: | CCTGTCCGTGGATGACTTTT |