| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.083110 |
| Chromosome: | chromosome 7 |
| Location: | 28997 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312150 | (1 of 1) PF00580//PF13361 - UvrD/REP helicase N-terminal domain (UvrD-helicase) // UvrD-like helicase C-terminal domain (UvrD_C) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTGGCACAAAACGCGAGTGTCACTAGCG |
| Internal bar code: | GTGTCTTCGCATTTAGTCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 75.7143 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAAGCTGCCTTGATTAGG |
| Suggested primer 2: | GTTGGCACCAAGCTAATGGT |