Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.083117 |
Chromosome: | chromosome 3 |
Location: | 4983458 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g180100 | UNG2 | Uracil DNA glycosylase; (1 of 1) 3.2.2.29 - Thymine-DNA glycosylase / Uracil/thymine DNA glycosylase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGGAAGGGTAGGACAGGGCCGCGGCAA |
Internal bar code: | GTTGACAGGTACTAGCTGTTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 304 |
LEAP-Seq percent confirming: | 99.3197 |
LEAP-Seq n confirming: | 438 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTACCATTCCACCATACCGC |
Suggested primer 2: | AGCACACCTCAATCAAAGGC |