| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083129 |
| Chromosome: | chromosome 10 |
| Location: | 5486947 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459000 | IBA57 | Putative transferase CAF17, mitochondrial; (1 of 1) K06980 - uncharacterized protein (K06980) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGCCCATACCCTGTGCCTCCTTCCTTT |
| Internal bar code: | GCAGGTGAGGTCCCCTCTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 52 |
| LEAP-Seq percent confirming: | 97.8873 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCCGGAAGTGGTGATAC |
| Suggested primer 2: | GCATTCTCGGAGTTGCTTTC |