| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.083130 |
| Chromosome: | chromosome 12 |
| Location: | 600618 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492500 | (1 of 39) IPR009003 - Peptidase S1, PA clan | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGATCGCAGTGGCTCCTGGCTGGCATC |
| Internal bar code: | CCGTAATGTGTCGGAGGCTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 199 |
| LEAP-Seq percent confirming: | 99.359 |
| LEAP-Seq n confirming: | 310 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGTTCTTCGCCATGCTC |
| Suggested primer 2: | CGTCAACATAGGACACCACG |