| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.083130 |
| Chromosome: | chromosome 12 |
| Location: | 600624 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492500 | (1 of 39) IPR009003 - Peptidase S1, PA clan | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGCAAACTCGCAGGGCGGCTTCACGC |
| Internal bar code: | TGCGTTCGACTTTTGTCTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 601 |
| LEAP-Seq percent confirming: | 99.6577 |
| LEAP-Seq n confirming: | 3494 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGTTCTTCGCCATGCTC |
| Suggested primer 2: | CGTCAACATAGGACACCACG |