| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.083136 |
| Chromosome: | chromosome 7 |
| Location: | 1501645 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323950 | PFH23,PHX23 | Putative prolyl 4-hydroxylase; (1 of 1) PTHR10869//PTHR10869:SF50 - PROLYL 4-HYDROXYLASE ALPHA SUBUNIT // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTAAATCACCCCCCCACCCCATAGACA |
| Internal bar code: | GAAGGTACTTCAACATGTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 156 |
| LEAP-Seq percent confirming: | 99.1501 |
| LEAP-Seq n confirming: | 350 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACATCCTGGTGTTTCCT |
| Suggested primer 2: | GTAGCACTAGATGAGGCGGC |