| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083140 |
| Chromosome: | chromosome 14 |
| Location: | 540657 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611552 | TGL22 | (1 of 32) 3.1.1.3 - Triacylglycerol lipase / Triglyceride lipase; Putative triacylglycerol lipase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGGGGGGGCGGCCCTGGGGAGGGGGC |
| Internal bar code: | CCCACTGAGAAAACCGGAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 257 |
| LEAP-Seq percent confirming: | 82.6255 |
| LEAP-Seq n confirming: | 428 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGCTTTCTTGTAGTCGC |
| Suggested primer 2: | CGACATGGGGTGTAAGTGTG |