Insertion junction: LMJ.RY0402.083157_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.083157
Chromosome:chromosome 11
Location:1725455
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467785 (1 of 3) PTHR10625:SF102 - HISTONE DEACETYLASE 10 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACAGCAACGCCCACGTCGCATCTCCCTC
Internal bar code:CCGAGCTTGACACACGGTTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:475
LEAP-Seq percent confirming:99.4948
LEAP-Seq n confirming:3939
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TATCCGTCAGTAACTCCGGC
Suggested primer 2:CGAGCAGCCAACATAGCATA