| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083229 |
| Chromosome: | chromosome 1 |
| Location: | 1745030 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009350 | AIH2 | Agmatine iminohydrolase; (1 of 2) 3.5.3.12 - Agmatine deiminase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGGGCGTGGGCGCGGGCAGCTTGATC |
| Internal bar code: | CCTTTCCGCCGCTGGATGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 711 |
| LEAP-Seq percent confirming: | 97.0616 |
| LEAP-Seq n confirming: | 89781 |
| LEAP-Seq n nonconfirming: | 2718 |
| LEAP-Seq n unique pos: | 185 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCCAGCTCGTCAGACTT |
| Suggested primer 2: | TTGTGACTGAGGAGTGCCTG |