| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.083232 |
| Chromosome: | chromosome 9 |
| Location: | 4194543 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394139 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGCACGCCCGTGGCCAGCGTCCTGCAG |
| Internal bar code: | CGGACGATACCGCCCCTCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 98.9151 |
| LEAP-Seq n confirming: | 9300 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAACTGCAGTGTGGTGAG |
| Suggested primer 2: | CCCACAGATTCACCTGGAGT |