Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.083239 |
Chromosome: | chromosome_4 |
Location: | 332652 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g217908 | FAP79 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCACGACCCTGACGGCTGGACCTGCCTAC |
Internal bar code: | ACTGCGCTAACGTCATGTCCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 292 |
LEAP-Seq percent confirming: | 95.7104 |
LEAP-Seq n confirming: | 9081 |
LEAP-Seq n nonconfirming: | 407 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTGTTGGCAGTCATACCCT |
Suggested primer 2: | ACTGACCATGCCATACGTGA |