Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.083244 |
Chromosome: | chromosome 2 |
Location: | 3761228 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g095300 | (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACATCTGCATGCCGCATACTCAAGTGTG |
Internal bar code: | CGCGGCGAGGACAGGGGCCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 720 |
LEAP-Seq percent confirming: | 98.7059 |
LEAP-Seq n confirming: | 2212 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTGCAACACCACCAATGAT |
Suggested primer 2: | TTCATTGGTGGTGCACACTT |