Insertion junction: LMJ.RY0402.083264_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.083264
Chromosome:chromosome 7
Location:573752
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g316600 MKP5 (1 of 3) K04459 - dual specificity MAP kinase phosphatase (DUSP, MKP); Dual-specificity protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAACCCTTTGCGCGCGCTAAATCAGGCC
Internal bar code:TAGGGGAGAGTGCGATGTGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:663
LEAP-Seq percent confirming:99.0933
LEAP-Seq n confirming:3388
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACGTGATAAGGGAGACG
Suggested primer 2:ACTGGTGTTCGATGTGGTGA