| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083303 |
| Chromosome: | chromosome 10 |
| Location: | 1185273 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426152 | MGL1 | Methionine gamma-lyase; (1 of 1) K01761 - methionine-gamma-lyase (E4.4.1.11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCGAACTCTCGTTTCCCGGCGTTCAGC |
| Internal bar code: | GGTTCGAAACTCCGCGCGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 99.1339 |
| LEAP-Seq n confirming: | 1259 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGTTCTCTTGGGCTGAC |
| Suggested primer 2: | TGTTTGCTGAAGGACTGACG |