| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.083306 |
| Chromosome: | chromosome 3 |
| Location: | 7251959 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCGCAGGCTGCGCGTATCCTCCAGGA |
| Internal bar code: | CTCCCGTTATATGTCATTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1084 |
| LEAP-Seq percent confirming: | 99.7149 |
| LEAP-Seq n confirming: | 5946 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGCAGGAAAGGGGTTA |
| Suggested primer 2: | TTTATGCGGTGTATGCGTGT |