| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083306 |
| Chromosome: | chromosome 3 |
| Location: | 7251969 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTCTACCTTGGTAGTGCGCCCGAGCCTT |
| Internal bar code: | AGGGAAACGGTAGTGTGGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 99.1697 |
| LEAP-Seq n confirming: | 4419 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGCAGGAAAGGGGTTA |
| Suggested primer 2: | TTTATGCGGTGTATGCGTGT |